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KMID : 0380020130280060394
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 6 p.394 ~ p.399
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Ethanol Extract of Astragalus membranaceus Bunge Induces Aquaporin-3 Expression in HaCaT Cells
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Park Hyun-Chul
Kim Hee-Taek
Ha Hun-Young
Lee Pyeong-Jae
Yoon Kyung-Sup
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Abstract
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Astragalus membranaceus Bunge is used in herbal medicine in Eastern Asian countries including Korea. In this study, we assessed the effects of A. membranaceus extract (AM) on the aquaporin-3 (AQP3) protein expression in HaCaT cells. AM did not affect viability of HaCaT cells. AQP3 expression and cell migration seem to be maximal at 100 μg/mL concentration. Epidermal growth factor receptor (EGFR) kinase inhibitor, PD153035, blocked AM-induced AQP3 expression and cell migration. In addition, an 80% ethanol extracts of herbal prescription, SinhyoTakleesan (ST), which is composed of A. membranaceus, Angelicae gigantis, Glycyrrhiza glabra Linne, and Lonicera japonica Flos also induced AQP3 expression at 20 μg/mL in HaCaT cells. Collectively, these results suggest that AM induce AQP3 expression via EGFR pathway.
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KEYWORD
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Astragalus membranaceus, SinhyoTakleesan, Aquaporin-3, Keratinocyte, EGFR
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